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Stable Cell Line Services
At BioArk Technologies, we specialize in constructing stable cell lines tailored to meet your specific research needs. Whether you’re working with cancer cell lines or adherent cell lines, we offer a range of services including gene overexpression, knockout, knock-in, and deletion.
Our affordable, reliable, and high-quality solutions will accelerate your research and deliver the results you need. Partner with us to drive innovation in your projects today!
Service Offerings:
Vector Construction
We offer comprehensive design and construction of plasmid vectors tailored to your genetic modification needs, with selectable markers for efficient screening. Our vector construction services include:
- Gene Overexpression:
- Targeted Gene Overexpression at AAVS1 or other safe harbor sites
- Inducible Gene Overexpression
- Gene Knockout via CRISPR
- Gene Deletion using double CRISPR cut sites
- Gene Tagging Services
Cell Line Transfection
We provide various transfection methods to suit different cell types and experimental requirements, including:
- Lipofection: Ideal for adherent cells.
- Electroporation: For difficult-to-transfect cell types.
- Viral Transduction: Leveraging lentiviruses or AAVs to efficiently deliver genetic material into target cells.
Cell Line Pool
Our services include the development of stable cell lines, customized to meet the unique needs of your project. We work with a wide variety of cell types, ensuring functional characteristics that align with your research goals.
Selection and Expansion
We apply selective pressure, such as antibiotic selection, to isolate successfully transfected cells. We then expand the selected clones in controlled environments to ensure consistency and stability.
Validation of Stable Cell Lines
We provide thorough characterization of generated cell lines, including:
- Genomic Analysis: Verification of gene integration through PCR or sequencing.
- Expression Analysis: Evaluation of target gene expression using techniques such as qPCR, Western blotting, or flow cytometry.