Call: 1-734-604-2386 Email: [email protected]
Lentivirus Package Services
Overview
Lentivirus packaging services provide researchers with high-efficiency lentiviral vectors for gene delivery into a wide range of target cells, including both dividing and non-dividing cells. Lentiviral vectors, derived from the Human Immunodeficiency Virus (HIV), are widely utilized in gene therapy, functional genomics, stable cell line development, and various research fields, including high-throughput gene function screening and transgenic animal model development.
At BioArk Technologies, we specialize in high-titer, ready-to-use lentiviral packaging designed for gene expression, knockdown, and CRISPR genome editing. Our service ensures:
✅ High transduction efficiency
✅ Low cytotoxicity
✅ Batch-to-batch consistency
Technology Background
1.) Three-Plasmid System (2nd Generation)
The most common method for lentivirus production, this system consists of:
- Transfer Plasmid
- Packaging Plasmid (Gag, Pol, and Rev integrated into a single vector)
- Envelope Plasmid
If you require 2nd-generation lentivirus packaging, please contact us at [email protected].
2. Four-Plasmid System (3rd Generation)
This system consists of:
- Transfer Plasmid
- Two Packaging Plasmids (one encoding Gag/Pol and another encoding Rev)
- Envelope Plasmid
While the 3rd-generation system enhances safety compared to the 2nd generation, it generally produces lower viral titers. By default, we use the 3rd-generation system, unless otherwise requested by the customer.
Service Offerings
1. Project Support
- Assistance in virus system selection and design
- Customization of lentiviral constructs to include specific genes, regulatory elements, or tags
2. Lentivirus Subcloning
To enhance functional detection, we offer gene subcloning into the following constructs:
- Lenti-mini GFP construct (~330bp): A compact mini-GFP tag used for rapid expression analysis post-transduction.
- Lenti-Selection Marker Constructs: Includes Puromycin, Blasticidin (BSD), Neomycin, etc., for stable cell line screening.
3. Lentivirus Packaging
- Production of high-titer lentiviral stocks using optimized protocols for maximum yield and purity
- Comprehensive quality control for titer, purity, and functionality
4. Functional Testing
Available assays based on construct type and project requirements, including:
✅ qPCR titer quantification
✅ Fluorescence detection
✅ Immunofluorescence analysis
✅ Western blot validation
5. Advanced Endotoxin Removal
- Our plasmids are endotoxin-free, significantly reducing endotoxin levels in viral preparations
- For industrial-scale production, we offer endotoxin removal using Endotoxin Removal Column Kits
For inquiries or custom requests, contact us at [email protected].
Pricing
| Catalog Number | Minimum Titer | Volume | 1st Tube Price | 2nd Tube Price | Turnaround |
|---|---|---|---|---|---|
| LPSVC-mini | >10⁷ TU/ml | 200 µl | $199 | $199 | 2-3 weeks |
| LPSVC-midi | >10⁸ TU/ml | 200 µl | $399 | $399 | 2-3 weeks |
| LPSVC-maxi | >10⁹ TU/ml | 200 µl | $699 | $699 | 2-3 weeks |
| Titer and Functional Service | Price | Note |
|---|---|---|
| qPCR | - | • Optimized reverse transcription quantitative PCR (RT-qPCR) is applied to quantify lentiviral RNA in viral particles |
| Fluorescent Tag Analysis | +$50 | • For proteins tagged with GFP, RFP, BFP or others • HEK293T cells with infected with the virus, and fluorescence imaging will be provided |
| Protein Tag Analysis (Immunofluorescence Staining) | +$70 | • For proteins tagged with Mcy, His, Flag or other ucstomized tags. • Immunofluorescence staining will be performed to detect expression after viral transduction |
| Customized Testing (Western Blot) | +$120 | • Suitable for untagged proteins or customized detection based on customer requirements. • Customers must provide the primary antibody for the assay. |
| Titer and Functional Service | Price | Note |
|---|---|---|
| Lenti-miniGFP Subcloning & Fluorescent Tag Analytis | +$199 | • Transfer the customer's lentivirus genes into our specialized lenti-miniGFP (330 bp) construct, with miniGFP expression analyzed through infection of HEK293T cells. |
| Lenti Selection Subcloning | +$189 | • Transfer the customer's lentivirus genes into our specialized Lenti Selection construct, allowing selection of Puro, BSD, Neo, or customized selection markers. |