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Overexpression Targeted Knock-In
This technique enables the precise integration of target genes or regulatory cassettes into safe harbor sites—genomic regions where foreign DNA can be inserted without disrupting essential endogenous gene functions or causing adverse cellular effects. These sites are widely used in genome engineering for stable gene insertion, ensuring long-term and predictable transgene expression.
Commonly used safe harbor sites include human AAVS1 and CCR5, as well as the mouse ROSA26 locus. Our standard kit utilizes the AAVS1 site as the default insertion locus. For alternative loci or custom services, please contact us at [email protected].
Technical Background
CRISPR-Cas9-mediated targeted knock-in at safe harbor sites involves:
- Cas9: An endonuclease that creates a double-strand break (DSB) at the target site.
- Guide RNA (gRNA): Directs Cas9 to the specific safe harbor locus, such as AAVS1 or the mouse ROSA26 locus.
- Donor DNA template: A construct containing the desired transgene flanked by homology arms complementary to sequences adjacent to the AAVS1 site, facilitating targeted insertion via homology-directed repair (HDR).
Key Features of Our Products
- Streamlined CRISPR and Donor Vectors
- Designed for efficient target gene integration, outperforming commercially available alternatives.
- Targeted vs. Random Integration
- Many conventional approaches rely on non-targeting lentiviruses, leading to random gene integration, which can pose safety risks and unpredictable outcomes, particularly in gene therapy and clinical research.
- Our vector and virus kits enable precise, targeted integration at safe harbor sites, significantly reducing these risks.
- User-Friendly Plasmid Kits for Broad Accessibility
- Unlike many market solutions that require electroporation—necessitating specialized equipment and techniques—our plasmid-based kits are optimized for ease of use, making them ideal for adherent cancer cell lines.
| Major Vector | Donor Vector | Scramble Control Vector | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Class | Product Name | SKU | Information | Donor Name | SKU | Information | Scramble Name | SKU | Information |
| Non-Viral CDS-P011k | CRISPR KN AIO Kit-Gene AAVS1, Vector type | COT-FXD00A-AAVS1gk | The CRISPR tool specifically designed to target and cut human AAVS1 safe harbor site. | AAVS1 Dnr Std Kit | TBD | Insert Customer Gene into human AAVS1 site, please specify your genes by clicking the button | GFP Donor Control AAVS1 Dnr Std Ctrl Kit, vector type | TBD | Insert GFP into human AAVS1 site as control |
| Non-Viral CDS-P011k | CRISPR KN AIO Kit-Gene ROSA26, Vector type | COT-FXD00A-ROSA26gk | The CRISPR tool specifically designed to target and cut mouse ROSA26 safe harbor site. | Dnr Std Kit-ROSA26 Site | TBD | Insert Customer Gene into mouse ROSA26 site, please specify your custom gene by clicking the button | GFP Donor Control Dnr Std Kit-ROSA26 Site, vector type | TBD | Insert GFP into mouse ROSA26 site as control |
Our design program assists customers in adjusting vector components and developing specific functions tailored to their unique requirements.
If you have any further inquiries regarding your project, please contact us. We are fully equipped to accommodate a wide range of your requirements.