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Genome Deletion
CRISPR Genome Knockout Deletion provides an efficient method for deleting genomic fragments of various sizes, ranging from short deletions to large deletions exceeding 10 kb. It is a valuable tool for studying the functions of non-coding regions of the genome, creating disease models such as Huntington’s disease, exploring gene cluster complexities, investigating chromatin architecture, and examining the role of pathogenic copy number variations (CNVs).
Technical Background
Key Components:
- Cas9 Protein: A nuclease that creates double-strand breaks (DSBs) at specified genomic locations, guided by a complementary RNA sequence.
- Guide RNA (gRNA): A synthetic RNA molecule designed to bind to a specific target sequence in the genome, directing the Cas9 protein to the desired site.
- DNA Repair Mechanisms: After the DSB is introduced by Cas9, the cell’s natural repair mechanisms take over. The primary repair pathways involved are:
- Non-Homologous End Joining (NHEJ): The predominant pathway for gene knockout, NHEJ can lead to insertions or deletions (indels) at the break site, causing frameshifts or premature stop codons that result in gene knockout.
- Homology-Directed Repair (HDR): Although not commonly used for knockout deletions, HDR can be applied for precise gene editing when a donor template is provided.
Features of Our Products
- Flexible Cloning Options: We offer three different cloning methods for the CRISPR deletion kit:
- Cas9 vector with a separate gRNA vector.
- Two distinct Cas9 + gRNA All-in-one vectors. Each vector carries one gRNA.
- A specialized vector designed for the simultaneous delivery of two gRNAs, allowing for the efficient removal of target genomic fragments.
User-Friendly Techniques: Our plasmid kits are designed to be lab-friendly, eliminating the need for electroporation and making them easy to use in most lab settings. These kits are particularly well-suited for adherent cancer cell lines.
Our design program assists customers in adjusting vector components and developing specific functions tailored to their unique requirements.
If you have any further inquiries regarding your project, please click the button to contact us. We are fully equipped to accommodate a wide range of your requirements.