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Overexpression Targeted Knock-In
CRISPR knockout (KO) is a revolutionary gene-editing technique that allows researchers to disrupt or “knock out” specific genes within an organism’s genome. The CRISPR-Cas9 system is the most widely used tool for this purpose, harnessing a natural bacterial defense mechanism against viral infections. Our technique provides a rapid and efficient approach to disrupt gene expression for both research and therapeutic applications.
Technical Background
- spCas9 Protein: An endonuclease that induces double-strand breaks (DSBs) in DNA at specific genomic loci.
- Guide RNA (gRNA): A short RNA sequence that guides the Cas9 protein to the target gene through complementary base pairing.
- Repair Pathways: Following the creation of a DSB, the cell’s repair machinery is activated. The primary repair pathways are:
- Non-Homologous End Joining (NHEJ): This repair mechanism often results in insertions or deletions (indels) at the break site, leading to frameshift mutations that can disrupt gene function.
- Homology-Directed Repair (HDR): Typically used for precise edits, HDR can also be employed when a donor template is provided. Although HDR is less common for knockout purposes, it can be leveraged to integrate exogenous DNA into the genome, facilitating quick screening for knockout stable cell lines.
By utilizing the NHEJ repair pathway, researchers can efficiently create gene knockouts, resulting in the loss of gene function. On the other hand, using HDR allows for precise integration of exogenous DNA fragments into the genome.
Features of Our Products
- Dual Repair Pathways: Our CRISPR knockout (KN) tool supports both NHEJ and HDR, offering flexibility depending on customer preferences and project requirements.
- Two Delivery Options: We provide two versions of our CRISPR KN tool: lentivirus and regular plasmid, allowing customers to choose the most suitable option for their needs.
Superior Knockout Efficiency: Our tools deliver enhanced knockout efficiency, leveraging the latest advancements in CRISPR technology, including optimized gRNA scaffold structures and improved gRNA sequence selection.
Our design program assists customers in adjusting vector components and developing specific functions tailored to their unique requirements.
| Major Vector | Scramble Control Vector | |||||
|---|---|---|---|---|---|---|
| Class | Product Name | SKU | Information | Scramble Name | SKU | Information |
| Non-Viral All-In-one COT-P031k | CRISPR KN AIO Kit | TBD | The non-viral CRISPR kit includes three AI-designed gRNA sites tailored to target the customer-specified genes. Please click button to design the target genes. | Scramble Control CRISPR KN AIO Ctrl Kit, Vector type | TBD | The scramble sequence are used as non-specific cutting control |
| Viral All-in-one COM-P032k | CRISPR KN Lenti-AIO Kit | TBD | The CRISPR lentivirus kit packages three AI-designed gRNA sites into a single mixture tube for precise targeting and cutting of customer-specified genes. Please click button to design the target genes. | Scramble Control CRISPR KN Lenti-AIO Ctrl Kit, lentivirus type | TBD | The scramble sequence are used as non-specific cutting control |
If you have any further inquiries regarding your project, please click the button to contact us. We are fully equipped to accommodate a wide range of your requirements.