This technique enables the precise integration of target genes or regulatory cassettes into safe harbor sites—genomic regions where foreign DNA can be inserted without disrupting essential endogenous gene functions or causing adverse cellular effects. These sites are widely used in genome engineering for stable gene insertion, ensuring long-term and predictable transgene expression.

Commonly used safe harbor sites include human AAVS1 and CCR5, as well as the mouse ROSA26 locus. Our standard kit utilizes the AAVS1 site as the default insertion locus. For alternative loci or custom services, please contact us at [email protected].